Effects of Lectin Preparations from Microgramma vacciniifolia Rhizomes on the Survival, Digestive Enzymes, and Acetylcholinesterase Activity of Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae)

In this study, we evaluated the susceptibility of Alphitobius diaperinus larvae and adults to saline extract (SE), lectin-rich fraction (FR), isolated lectin (MvRL) from Microgramma vacciniifolia rhizomes. To determine immediate effects, were exposed SE (10.5 mg/mL), FR (7.5 or MvRL (1.0 mg/mL) for 48 h. Live insects acetylcholinesterase (AChE) activity. The delayed effects (0.2 0.4 checked by incubating 16 days with a diet containing preparations. vitro on gut digestive enzymes investigated. All preparations showed larvicidal effect but had no adult survival. Extracts FR-treated higher AChE activity than control insects. assay, lost biomass after consuming FR. was most effective inhibitory agent trypsin-like amylase activities (88% 65% inhibition, respectively). inhibited endoglucanase in 94–98%, while exoglucanase 93.2 94.1%, respectively. conclusion, M. rhizomes contain compounds (including MvRL) that affect survival physiology A. diaperinus, acting as potential natural insecticides controlling pest.